Chromosome-level genome assembly of black carp Mylopharyngodon piceus using Nanopore and Hi-C technologies

作者：Yuxuan Zhang, Yanwen Shao, Xudong Liu, Liang Zhong, Zhaoyan Zhong, Weiwei Zeng, Jiping Zhang, Wenlong Cai, Runsheng Li · 发表于：Scientific Data · 年份：2025 · DOI：10.1038/s41597-025-04421-1 · 研究领域：Genomics and Phylogenetic Studies、CRISPR and Genetic Engineering、Advanced biosensing and bioanalysis techniques

Black carp (Mylopharyngodon piceus) is one of the "four famous domestic fishes" in China and an important economic fish in freshwater aquaculture. A high-quality genome is essential for advancing future biological research and breeding programs for this species. In this study, we aimed to generate a high-quality chromosome-level genome assembly of black carp using Nanopore and Hi-C technologies. The final genome assembly was 848.70 Mb in length, with a contig N50 of 3.37 Mb and a scaffold N50 of 34.13 Mb. The genome was anchored onto 24 chromosomes by Hi-C technology. The BUSCO (Benchmarking Universal Single-Copy Orthologs) completeness score of the genome assembly is 97.6% when compared to the Actinopterygii_odb10 database. In total, 203.54 Mb of repeat sequences and 37,418 protein-coding genes were predicted in the genome assembly. Taken together, our study provides a chromosome-level genome assembly, which can serve as a valuable genetic resource to support further biological studies and breeding efforts of black carp.