Comparative transcriptome analysis reveals molecular mechanisms of resistance in Chinese cabbage to Plasmodiophora brassicae pathotype 11

作者：Yue Qiu, Jinhao Zhang, Chunju Deng, Jiasheng Yuan, Bowen Wang, Han Meng, Mohamed Mohany, Liting Zeng, Lanfang Wei, Waqar Ahmed, Guanghai Ji · 发表于：Frontiers in Microbiology · 年份：2025 · DOI：10.3389/fmicb.2025.1495243 · 被引用次数：5 · 研究领域：Plant Disease Resistance and Genetics、Plant and Fungal Interactions Research、Plant-Microbe Interactions and Immunity

Background and aims Clubroot caused by the soilborne obligate parasite Plasmodiophora brassicae , is a devastating disease of Chinese cabbage and other crucifers. The innate diversity and adaptability of this pathogen pose significant challenges to effective control measures. However, the varied response mechanisms exhibited by hosts to pathotype 11 at a molecular level are still unclear. Methods and results This study investigated the resistance response and underlying molecular mechanism of two Chinese cabbage ( Brassica rapa ) varieties (JP and 83-1) to P. brassicae pathotype 11 through comparative transcriptome analysis and microscopic study. Results demonstrated that 14 days after inoculation (dai) is a critical time point of the infection process for resistant variety to inhibit the proliferation of P. brassica . Although the highly resistant variety JP did not exhibit a complete immune response to pathotype 11, it demonstrated a significant resistance level against P. brassicae pathotype 11 by restricting its proliferation in the xylem vessels. Microscopic analysis at 21 dai revealed that the resistant cultivar (JP) root structure remained largely unaffected, while the roots of the susceptible cultivar (83-1) exhibited significant tissue distortion and gall formation, underscoring the effectiveness of the resistance mechanisms. Comparative transcriptome analysis revealed substantial differences in the number and types of differentially expressed genes (DEGs) between th...