Seed microbiomes promote Astragalus mongholicus seed germination through pathogen suppression and cellulose degradation

作者：Da Li, Weimin Chen, Wen Luo, Haofei Zhang, Yang Liu, Duntao Shu, Gehong Wei · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-024-02014-5 · 被引用次数：28 · 研究领域：Plant-Microbe Interactions and Immunity、Biocrusts and Microbial Ecology、Plant Stress Responses and Tolerance

BACKGROUND: Seed-associated microorganisms play crucial roles in maintaining plant health by providing nutrients and resistance to biotic and abiotic stresses. However, their functions in seed germination and disease resistance remain poorly understood. In this study, we investigated the microbial community assembly features and functional profiles of the spermosphere and endosphere microbiomes related to germinated and ungerminated seeds of Astragalus mongholicus by using amplicon and shotgun metagenome sequencing techniques. Additionally, we aimed to elucidate the relationship between beneficial microorganisms and seed germination through both in vitro and in vivo pot experiments. RESULTS: Our findings revealed that germination significantly enhances the diversity of microbial communities associated with seeds. This increase in diversity is driven through environmental ecological niche differentiation, leading to the enrichment of potentially beneficial probiotic bacteria such as Pseudomonas and Pantoea. Conversely, Fusarium was consistently enriched in ungerminated seeds. The co-occurrence network patterns revealed that the microbial communities within germinated and ungerminated seeds presented distinct structures. Notably, germinated seeds exhibit more complex and interconnected networks, particularly for bacterial communities and their interactions with fungi. Metagenome analysis showed that germinated seed spermosphere soil had more functions related to pathogen inhibi...