Effects of green manuring on chemical characteristics and microecology of tobacco-growing soil in central henan

作者：Wei Liu, Xiaolong Chen, Yuanyuan Zhao, Hongzhi Shi · 发表于：BMC Microbiology · 年份：2025 · DOI：10.1186/s12866-025-03742-w · 被引用次数：5 · 研究领域：Soil Carbon and Nitrogen Dynamics、Mycorrhizal Fungi and Plant Interactions、Agriculture, Soil, Plant Science

OBJECTIVE: This study explored green manuring effects on microecology, carbon/nitrogen levels, and enzyme activity in tobacco-growing soils. METHODS: After 30,000 kg·hm⁻² overpressure and 28 days of natural decomposition, plants (Hordeum vulgare L. (DM), Secale cereale L. (HM), Medicago sativa L. (MX), Vicia dasycarpa Ten. (SZ), Brassica napus L. (YC), and Astragalus sinicus L. (ZY)) were grown in a lab to analyze changes in carbon and nitrogen components, microbial community structure, and enzyme activities in the soil. RESULTS: The results showed improved soil nutrition and increased organic matter, nitrate-nitrogen, and ammonium-nitrogen levels. Except for MX, the other treatments markedly enhanced carbon/nitrogen in the soil microbial biomass. Compared to the CK treatment, the HM treatment boosted alkaline phosphatase and catalase activities by 20.54% and 61.98%, respectively, and increased the soil microbial biomass carbon and nitrogen content by 99.59% and 61.07%, respectively. Green manuring altered bacterial and fungal community structures. Proteobacteria, Actinobacteria, Chloroflexi, Firmicutes, Acidobacteria, Planctomycotes, and Gemmatimonadetes were the dominant bacterial communities. MX significantly increased the abundance of Bacteroidetes. Over half of all soil fungi were Ascomycota, with SZ substantially increasing their abundance. LEfSe analysis revealed 29 differential bacterial and fungal species (LDA > 3.5); fungi comprised 29 distinct species in six treatm...