Telomere‐to‐telomere genome of the desiccation‐tolerant desert moss Syntrichia caninervis illuminates Copia ‐dominant centromeric architecture

作者：Bei Gao, Jichen Zhao, Xiaoshuang Li, Jianhua Zhang, Melvin J. Oliver, Daoyuan Zhang · 发表于：Plant Biotechnology Journal · 年份：2025 · DOI：10.1111/pbi.14549 · 被引用次数：10 · 研究领域：Bryophyte Studies and Records、Biocrusts and Microbial Ecology、Lichen and fungal ecology

The extremophile desert moss Syntrichia caninervis, from the Gurbantunggut Desert in China, was capable of surviving simulated Mars conditions (Li et al., 2024). Syntrichia caninervis has become a research model for plant desiccation tolerance (Oliver et al., 2020). The chromosome-level genome of S. caninervis, from gametophytes originating from the Mojave Desert, was sequenced and assembled (Silva et al., 2021), facilitating research on gene function (Li et al., 2023) and comparative and evolutionary genomics (Zhang et al., 2024). This S. caninervis genome was considered an initial version (ScMoj v1). Because the ScMoj v1 genome relies on assembly of short reads, it has issues with continuity, gaps and assembly errors related to repetitive sequences. Here we generated a high-quality genome for the S. caninervis isolated from the Gurbantunggut Desert (designated ScGur). Cultured gametophytes propagated from a single female gametophyte (Figure S1) were used for DNA isolation. The genome was assembled from PacBio High Fidelity (HiFi) and Oxford Nanopore Technologies (ONT) ultra-long reads (Table S1) using hifiasm and NextDenovo softwares. The complete circular genomes of the S. caninervis chloroplast (123 124 bp, Figure S2) and mitochondria (108 309 bp, Figure S3) were obtained using GetOrganelle. A single circular bacterial genome of 6 933 718 bp (Figure 1a) was discovered during assembly with high genomic synteny (Figure 1b) to three genomic contigs of Paenibacillus cellulosi...