Identification and functional analysis of energy metabolism and pyroptosis-related genes in diabetic nephropathy

作者：Shan He, Jian Ye, Yu Wang, Lu Xie, Si Yi Liu, Qin Chen · 发表于：Heliyon · 年份：2025 · DOI：10.1016/j.heliyon.2025.e42201 · 被引用次数：3 · 研究领域：Inflammasome and immune disorders、Chronic Kidney Disease and Diabetes、GDF15 and Related Biomarkers

Background: Energy metabolism and pyroptosis are integral to the pathogenesis of diabetic nephropathy (DN). However, the precise roles of energy metabolism and pyroptosis in DN development remain unclear. This study aims to elucidate the roles of energy metabolism- and pyroptosis-related differentially expressed genes (EMAPRDEGs) in DN development. Methods: EMAPRDEGs were identified by querying the GeneCards and Gene Expression Omnibus (GEO) databases. Subsequent analyses included Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, Gene Set Enrichment Analysis (GSEA), and Protein-Protein Interaction (PPI) network analysis. Additionally, mRNA-miRNA, mRNA-drug, and mRNA-transcription factor (TF) interaction networks were constructed. Differential expression and receiver operating characteristic (ROC) curve analyses were performed to evaluate the diagnostic potential of EMAPRDEGs. Immune cell infiltration in DN was assessed using the ssGSEA algorithm, and the expression levels of EMAPRDEGs in DN tissues were validated by quantitative real-time PCR (qRT-PCR). Results: , which were consistent with the bioinformatics predictions. Conclusion: emerge as potential biomarkers for diabetic nephropathy.