Engineered Extracellular Vesicles from Antler Blastema Progenitor Cells: A Therapeutic Choice for Spinal Cord Injury

作者：Shijie Yang, Borui Xue, Yongfeng Zhang, Haining Wu, Beibei Yu, Shengyou Li, Teng Ma, Xue Gao, Yiming Hao, Lingli Guo, Qi Liu, Xueli Gao, Yujie Yang, Zhenguo Wang, Mingze Qin, Yunze Tian, Longhui Fu, Bisheng Zhou, Luyao Li, Jianzhong Li, Shouping Gong, Bing Xia, Jinghui Huang · 发表于：ACS Nano · 年份：2025 · DOI：10.1021/acsnano.4c10298 · 被引用次数：8 · 研究领域：Extracellular vesicles in disease、Autophagy in Disease and Therapy、Viral Infections and Vectors

High Resolution Image Download MS PowerPoint Slide Deer antler blastema progenitor cells (ABPCs) are promising for regenerative medicine due to their role in annual antler regeneration, the only case of complete organ regeneration in mammals. ABPC-derived signals show great potential for promoting regeneration in tissues with limited natural regenerative ability. Our findings demonstrate the capability of extracellular vesicles from ABPCs (EVs ABPC ) to repair spinal cord injury (SCI), a condition with low regenerative capacity. EVs ABPC significantly enhanced the proliferation of neural stem cells (NSCs) and activated neuronal regenerative potential, resulting in a 5.2-fold increase in axonal length. Additionally, EVs ABPC exhibited immunomodulatory effects, shifting macrophages from M1 to M2. Engineered with activated cell-penetrating peptides (ACPPs), EVs ABPC significantly outperformed EVs from rat bone marrow stem cells (EVs BMSC ) and neural stem cells (EVs NSC ), promoting a 1.3-fold increase in axonal growth, a 30.6% reduction in neuronal apoptosis, and a 2.6-fold improvement in motor function recovery. These findings support ABPC-derived EVs as a promising therapeutic candidate for SCI repair.