Effects of ultra-processed foods on the liver: insights from gut microbiome and metabolomics studies in rats

作者：Liping Shi, Zhuoyuan Li, Xiaojun Ma, Junru Wang, Yueping Wu, Yongbin Zhu, Yanrong Wang, Yue Yang, Minxiu Luo, Jiangping Li, Xian Sun, Shulan He · 发表于：Frontiers in Nutrition · 年份：2025 · DOI：10.3389/fnut.2024.1503879 · 被引用次数：6 · 研究领域：Consumer Attitudes and Food Labeling、Biochemical Analysis and Sensing Techniques、Food composition and properties

Purpose High consumption of Ultra-processed foods (UPF) have been identified as a potential risk factor for Non-alcoholic fatty liver disease (NAFLD). Nevertheless, there is limited empirical evidence regarding the impact of UPF, which are typical combination of processed foods, on liver health through alterations in gut microbiota and metabolic processes. We aim to examine the potential impact of UPF on liver health and to explore the role of gut microbiota and metabolites. Methods This study used Sprague–Dawley rats to mimic modern UPF diets for 90 days. Some serum biochemical indices, inflammatory factors, oxidative stress markers, hematoxylin–eosin (HE) staining of the liver, 16S ribosomal RNA (rRNA) and Liquid chromatography-mass spectrometry (LC–MS) of rat feces were detected. Results The UPF diet-induced simple steatosis of the liver in rats without affecting the levels of IL-6, GSH, MDA, and SOD. Additionally, it modified the gut microbiota, increasing potentially harmful bacteria, such as norank_f__Desulfovibrionaceae and Staphylococcus , while also elevating the relative abundance of potentially beneficial bacteria, including Dubosiella and Allobaculum . Furthermore, the consumption of UPF led to a metabolomic disorder characterized by disruptions in the sphingolipid signaling pathway, sulfur relay system, and arachidonic acid metabolism. Conclusion In conclusion, the findings of this study indicate that the consumption of UPF influences the development of simple he...