Multifunctional Nanozyme with Aptamer-Based Ratiometric Fluorescent and Colorimetric Dual Detection of Prostate-Specific Antigen

作者：Peng Liu, L. Du, Fang Luan, Chuanwei Shi, Yeping Liu, Zhexu Gai, Fei Yang, Yanzhao Yang · 发表于：ACS Applied Materials & Interfaces · 年份：2025 · DOI：10.1021/acsami.4c22799 · 被引用次数：15 · 研究领域：Advanced Nanomaterials in Catalysis、Nanocluster Synthesis and Applications、Carbon and Quantum Dots Applications

The adsorption of DNA probes onto nanomaterials represents a promising bioassay technique, generally employing fluorescence or catalytic activity to generate signals. A significant challenge is maintaining the catalytic activity of chromogenic catalysts during detection while enhancing accuracy by overcoming the limitations of single-signal transmission. This article presents an innovative multimodal analysis approach that synergistically combines the oxidase-like activity of Fe-N-C nanozyme (Fe-NC) with red fluorescent carbon quantum dots (R-CQDs), further advancing the dual-mode analysis method utilizing R-CQDs@Fe-NC. In this system, R-CQDs integrate with Fe-NC to provide a steady reference red fluorescence signal, while Fe-NC serves as the catalytic active site. The adsorption of 6-carboxyfluorescein-labeled aptamers (FAM-apt) significantly enhanced the electron transfer capability of R-CQDs@Fe-NC, enhancing its catalytic performance and resulting in increased oxidation of 3,3',5,5'-tetramethylbenzidine (TMB). Concurrently, the green fluorescence of FAM-apt diminishes due to energy competition, photoinduced electron transfer, and the internal filtration effect by R-CQDs@Fe-NC, while the red fluorescence from R-CQDs@Fe-NC remains stable. Upon recognizing and binding to prostate-specific antigen (PSA), FAM-apt detaches from the surface of R-CQDs@Fe-NC. This leads to simultaneous variations in both the fluorescence signal of the system and the colorimetric signal of TMB. Base...