Heterologous Biosynthesis of Terpenoids in Saccharomyces cerevisiae

作者：Junyang Wang, Xu Ji, Yi Rong, D. Li, Xiaolin Shen, Zihe Liu, Y. Xia, Shuobo Shi · 发表于：Biotechnology Journal · 年份：2025 · DOI：10.1002/biot.202400712 · 被引用次数：25 · 研究领域：Plant biochemistry and biosynthesis、Microbial Natural Products and Biosynthesis、Microbial Metabolic Engineering and Bioproduction

Terpenoids are widely distributed in nature and have various applications in healthcare products, pharmaceuticals, and fragrances. Despite the significant potential that terpenoids possess, traditional production methods, such as plant extraction and chemical synthesis, face challenges in meeting current market demand. With the advancement of synthetic biology and metabolic engineering, it becomes feasible to construct efficient microbial cell factories for large-scale production of terpenoids. This article primarily centers on the heterologous expression of terpenoids in Saccharomyces cerevisiae, detailing the expression of terpenoid biosynthesis pathways through the utilization of cellular microcompartments, strategies for the efficient expression of key P450 enzymes in the synthesis pathway, and the regulation and optimization of host metabolism to enhance flux to terpenoids synthesis. Additionally, we analyze current challenges and propose solutions to further refine yeast chassis for more effective terpenoids production.