Characterization and fungicides sensitivity of Colletotrichum species causing Hydrangea macrophylla anthracnose in Beijing, China

作者：Juan Zhao, Yanli Cheng, Yayong Liu, Xiaojing Shi, Taotao Zhang, Wentao Qin · 发表于：Frontiers in Plant Science · 年份：2025 · DOI：10.3389/fpls.2024.1504135 · 被引用次数：6 · 研究领域：Plant Pathogens and Fungal Diseases、Plant-Microbe Interactions and Immunity、Mycorrhizal Fungi and Plant Interactions

Hydrangea macrophylla (Thunb.) Ser. is one of the widely cultivated plants in home gardens and scenic areas of China. Anthracnose disease is commonly observed during the normal growth of H. macrophylla , significantly impacting its ornamental and economic values. From 2021 to 2023, an investigation on H. macrophylla anthracnose was carried out in nine parks of Beijing, China, and a total of 114 Colletotrichum isolates were obtained from the diseased leaves with typical anthracnose symptoms. Based on morphological characteristics and phylogenetic analysis of six genomic loci including rDNA-ITS, ACT , TUB2 , CAL , CHS-1 , and GAPDH , these isolates were identified as belonging to six Colletotrichum species. Among which, C. gloeosporioides was the most abundant (65 isolates, 57.0%), followed by C. fructicola (33 isolates, 28.9%), C. aenigma (8 isolates, 7.0%), C. truncatum (4 isolates, 3.5%), C. subacidae (2 isolates, 1.8%) and C. sojae (2 isolates, 1.8%). Pathogenicity test conducted on detached leaves of H. macrophylla revealed a distinct variation in virulence among isolates from different Colletotrichum species, and wounding was either essential or conducive to successful infection. Specifically, C. gloeosporioides exhibited greater aggressiveness, resulting in larger lesions, while C. subacidae induced lesions most quickly. Fungicide sensitivity assays demonstrated that prochloraz exerted a remarkable inhibitory effect on the mycelial growth of representative isolates belon...