The Histone Lysine Demethylase KDM7A Contributes to Reward Memory via Fscn1‐Induced Synaptic Plasticity in the Medial Prefrontal Cortex

作者：Zhuojin Yang, Dongyu Yu, Feifei Gao, Danya Zhou, Yanan Wu, Xixi Yang, Jie Chen, Jing‐Si Yang, Mingshuai Shen, Yu-Xiang Zhang, Lai Wei, Chunxia Yan · 发表于：Advanced Science · 年份：2025 · DOI：10.1002/advs.202405352 · 被引用次数：6 · 研究领域：Epigenetics and DNA Methylation、Tryptophan and brain disorders、Genetics and Neurodevelopmental Disorders

Lysine demethylase 7A (KDM7A) catalyzes the removal of dimethylation from histone H3 lysine 9 and lysine 27, both of which are associated with transcription repression. Previous study indicates that Kdm7a mRNA in the medial prefrontal cortex (mPFC) increases after drug exposure, yet its role in drug-related behaviors is largely unknown. In a morphine-conditioned place preference (CPP) paradigm, these findings reveal a specific increase of Kdm7a expression in the mPFC 7 days after drug withdrawal. Subsequently, these results demonstrate that knockdown of Kdm7a in the mPFC do not affect the acquisition of morphine-induced CPP, but it attenuate memory consolidation. To further explore Kdm7a-mediated transcriptomic changes, this work employs Nanopore direct RNA sequencing. Transcriptome profiling unveils several gene expression alterations impacted by KDM7A, which are enriched in relevant neural function categories. Notably, this work identifies and validates fascin actin-bundling protein 1 (Fscn1) as a downstream molecular target. Knockdown of Fscn1 has a similar impact on CPP to Kdm7a, along with corresponding decrease of dendritic spine density and neuronal activity in the mPFC. Additionally, silencing Kdm7a decreases enrichment of H3K9me2 and H3K27me2 at the Fscn1 promoter region, suggesting that KDM7A may act as a crucial regulator of transcriptional responses to morphine-related reward memory via Fscn1.