Single-cell RNA sequencing of the carotid artery and femoral artery of rats exposed to hindlimb unloading

作者：Chengfei Li, Yikai Pan, Yuan Wang, Yuan Wang, Xi Li, Yateng Tie, Shuhan Li, Ruonan Wang, Xingcheng Zhao, Xianchun Yan, Xianchun Yan, Yongchun Wang, Yongchun Wang, Xiqing Sun · 发表于：Cellular and Molecular Life Sciences · 年份：2025 · DOI：10.1007/s00018-024-05572-x · 被引用次数：5 · 研究领域：Spaceflight effects on biology、Single-cell and spatial transcriptomics、Angiogenesis and VEGF in Cancer

BACKGROUND: Prolonged spaceflight is known to cause vascular deconditioning and remodeling. Tail suspension, a widely used spaceflight analog, is reported to result in vascular remodeling of rats. However, little is known about the cellular atlas of the heterogeneous cells of CA and FA from hindlimb-unloaded rats. METHODS: Firstly, we leveraged scRNA-seq to perform clustering analysis to identify diverse cell populations and sub-clusters within CA and FA from rats subjected to 3 months of hindlimb unloading. The dysregulated genes specific for artery types and cell types in HU group compared to Con were unraveled. Then R package "Cellchat" was used to reveal ligand-receptor cellular communication. At last, the TF network analysis was performed using the SCENIC R package to predict the pivotal TFs in rat artery remodeling induced by hindlimb unloading. RESULTS: Clustering analysis identified ECs, SMCs, fibroblasts, and a spectrum of immune cells, as well as neuronal and stem cells. Notably, an increased percentage of ECs in the CA and a diminished proportion of SMCs in both CA and FA were observed following tail suspension. Intersection of dysregulated genes specific for artery type and cell type after tail suspension revealed several gene sets involved in ECM remodeling, inflammation, vasoconstriction, etc. Fibroblasts, in particular, exhibited the most significant gene expression variability, highlighting their plasticity. Subclustering within ECs, SMCs and fibroblasts revea...