Multi-omic analysis to develop key genes involved in carotenoid synthesis and establish indicative molecular markers for oil palm fruit colour

作者：Ruimin Zhang, Xiaoyu Liu, Cheng Yang, Jerome Jeyakumar John Martin, Xinyu Li, Lixia Zhou, Dengqiang Fu, Qihong Li, Rui Li, Hongxing Cao · 发表于：Industrial Crops and Products · 年份：2025 · DOI：10.1016/j.indcrop.2025.120487 · 被引用次数：6 · 研究领域：Oil Palm Production and Sustainability、Cocoa and Sweet Potato Agronomy、Plant biochemistry and biosynthesis

Oil palm (Elaeis guineensis Jacq.) is an important tropical woody oil-bearing economic crop. The outer skin of the fruit is rich in carotenoids, which are not only involved in the formation of oil palm skin color, but also confer antioxidative, anti-aging, and immune-regulating properties to palm oil. In this study, outer skin of virescens oil palm fruits (CS) from O×G Amazon and nigrescens oil palm fruits (CT) from Deli×Ghana as experimental materials after pollination at 95 days (CS1, CT1), 125 days (CS2, CT2), and 185 days (CS3, CT3). The changes in total carotenoid content and differences in metabolome and transcriptome at different developmental stages were analyzed, and SNPs mutation sites of related genes were selected to develop KASP molecular markers that can distinguish virescens and nigrescens oil palm. The results showed that the outer skin content of two species of oil palms varied significantly in terms of the total carotinoid content at various developmental stages. The virescens oil palm variety was found to contain a diverse range of carotenoids, with a total of 48 types identified. Similarly, the nigrescens oil palm variety was found to contain 52 types of carotenoids. The six types with the highest carotinoid content were: α-Carotenoids (C1), lycopenes (C2) γ-Carotenoids (C4) β-Carotenoids (C5), lycopene (C6), lutein (C38); Preliminary identification of 9 candidate genes in the carotenoid synthesis pathway of oil palm peel. In CS and CT, the PSY2 gene, PDS,...