Polyphenols From Morchella sextelata Induce Apoptosis of Colorectal Cancer Cells Through ROS‐Mediated Endogenous Mitochondrial Apoptosis Pathway In Vitro

作者：Feihong Zhai, Yan Wang, Miaoqing Yan · 发表于：Journal of Food Biochemistry · 年份：2025 · DOI：10.1155/jfbc/7777790 · 被引用次数：6 · 研究领域：Fungal Biology and Applications、Medicinal Plants and Bioactive Compounds、Genomics, phytochemicals, and oxidative stress

Colorectal cancer (CRC) is a common malignancy of the digestive tract. Although chemotherapy is considered the most effective method for the treatment of CRC, these drugs have significant side effects. The identification of antitumour active ingredients with high efficiency and fewer toxic side effects from natural products is important. The effects of polyphenols from artificially cultivated Morchella sextelata (MSP) on CRC were analysed at the cellular level. The antiproliferative and proapoptotic effects of MSP on HCT 116 and HT‐29 CRC cells were determined by a 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide (MTT) assay, a colony formation assay, morphological observation and flow cytometry. The anti‐CRC effects and their molecular mechanism were subsequently explored by RT‐qPCR, Western blotting, mitochondrial membrane potential assays, reactive oxygen species (ROS) level assays and antioxidant enzyme system assays. MSP had dose‐dependent cytotoxic effects as revealed by the inhibition of colony formation and induction of morphological changes and apoptosis in HCT 116 and HT‐29 cells. The RT‐qPCR results revealed that MSP treatment decreased the expression of Bcl-2 and upregulated the expression of Bax, caspase-3 and caspase-9 , which were verified by Western blot experiments. Furthermore, MSP led to the destruction of the mitochondrial membrane potential, which suggested that MSP induced mitochondria‐mediated apoptosis in these two cell types. In addition, ...