Enhancing single-cell transcriptomics using interposed anchor oligonucleotide sequences

作者：Jianfeng Sun, Martin Philpott, Danson Loi, Gabriela Hoffman, Jonathan A. Robson, Neelam Mehta, Eleanor Calcutt, Vicki Gamble, Tom Brown, Tom Brown, Udo Oppermann, Adam P. Cribbs · 发表于：Communications Biology · 年份：2025 · DOI：10.1038/s42003-025-07474-5 · 被引用次数：5 · 研究领域：Single-cell and spatial transcriptomics、Advanced biosensing and bioanalysis techniques、Extracellular vesicles in disease

Single-cell transcriptomics, which utilises barcodes and unique molecular identifiers (UMIs) for polyA+ mRNA capture, is compromised by oligonucleotide synthesis errors. To address this, we modified the oligonucleotide capture design and integrated an interposed anchor between the barcode and the UMI. This design significantly reduces the need to discard reads due to synthesis inaccuracies. Our results demonstrate that this anchor-enhanced design substantially improves gene expression profiles in droplet-based single-cell sequencing analyses.