G462S substitution of AaCYP51 confers moderate resistance to tebuconazole in Alternaria alternata

作者：Feng Ji, Liu‐Ti Cai, Tao Li, Hancheng Wang, Chuanqing Zhang · 发表于：Pest Management Science · 年份：2025 · DOI：10.1002/ps.8654 · 被引用次数：12 · 研究领域：Fungal Plant Pathogen Control、Fungal and yeast genetics research、Entomopathogenic Microorganisms in Pest Control

BACKGROUND: Tobacco brown spot (TBS) caused by Alternaria alternata is one of the most common diseases of tobacco in China, resulting in large loss in yield and quality. Demethylation inhibitors (DMIs) such as tebuconazole are commonly used pesticides to control TBS. However, their control effect has shown a downward trend in recent years. In this study, the occurrence and molecular mechanism of resistance to tebuconazole in Alternaria alternata were analyzed. RESULTS: The resistance of 63 strains of Alternaria alternata to tebuconazole was investigated with the concentration of 5 and 20 μg/mL as the identification standard, and the resistance frequency was as high as 93.65%. It was found that the target mutation from G to S at the 462nd amino acid position of CYP51 was the cause of moderate resistance to tebuconazole in A. alternata. Molecular docking analysis further confirmed that the G462S mutation of AaCYP51 decreased the binding affinity of tebuconazole to CYP51. The artificial AaCYP51-G462S transformants based on wild-sensitive GZA-24 showed resistance to tebuconazole and cross-resistance to metconazole and prothioconazole. In the present investigation, the virulence of the CYP51-G462S mutant was reduced, while mycelial growth, sporulation, and conidial germination did not change in comparison with the progenitor strain GZA-24. In addition, the mutants containing the G462S mutation in AaCYP51 exhibited decreased sensitivity to high osmotic stress stimulated by 1 M NaCl...