Overexpression of the GmPM35 Gene Significantly Enhances Drought Tolerance in Transgenic Arabidopsis and Soybean

作者：Xinyu Wang, Yao Sun, Rui Wang, Xinyang Li, Yongyi Li, Tianyu Wang, Zongxin Guo, Yan Li, Wenxi Qiu, Shuyan Guan, Qi Zhang, Piwu Wang, Mingze Li, Siyan Liu, Xuhong Fan · 发表于：Agronomy · 年份：2025 · DOI：10.3390/agronomy15010192 · 被引用次数：13 · 研究领域：Soybean genetics and cultivation、Plant Genetic and Mutation Studies、Plant tissue culture and regeneration

Drought stress is one of the major adversity stresses affecting soybean (Glycine max [L.] Merr.) yield. Late embryogenesis abundant protein (LEA protein) is a large family of proteins widely distributed in various types of organisms, and this class of proteins plays an important role in protecting proteins, membrane lipids, and lipids inside the cell. The soybean GmPM35 gene is a member of the LEA_6 subfamily. The expression of the GmPM35 gene was significantly increased after drought stress in soybeans. A subcellular localization assay confirmed that the gene acts on the cell membrane. Against wild-type Arabidopsis thaliana, we found that Arabidopsis lines overexpressing the GmPM35 gene were significantly more drought-tolerant at germination and seedling stages under drought stress. To further investigate the drought tolerance function of this gene in soybeans, nine overexpression lines of the T3 generation soybean GmPM35 gene and two editing lines of the T3 generation soybean GmPM35 gene were obtained by Agrobacterium-mediated method using a wild-type soybean strain (JN28) as a receptor. Germination rate, root length, chlorophyll (CHL) content, Proline (Pro) content, malondialdehyde (MDA) content, superoxide anion (O2•−) content, hydrogen peroxide (H2O2) content, (NBT, DAB) staining, and activities of antioxidant enzymes (CAT, SOD, POD), and photosynthetic physiological indexes of the three different types of strains were measured and analyzed before and after drought stres...