Prevalence of Site-Specific Mycoplasma genitalium Infection and Macrolide and Fluoroquinolone-Associated Mutations in Men Who Have Sex with Men in Shenzhen, China

作者：Xinying Leng, Rui Zhu, Xian Ao, Ying Zhou, Kechun Zhang, Hu Tian, Jiaxin Wu, Zhaoqi Chen, Lixia Huang, Nanxuan Huang, Xinyuan Li, Ruaa Ahmed Alnour, Zhengyu Xue, Xiangcai Zhang, Han Liu, Tuerhongjiang Axirejiang, Wujian Ke, Huachun Zou · 发表于：Infection and Drug Resistance · 年份：2025 · DOI：10.2147/idr.s489403 · 被引用次数：1 · 研究领域：Reproductive tract infections research、Pharmaceutical and Antibiotic Environmental Impacts、HIV/AIDS Research and Interventions

Background: Mycoplasma genitalium (MG) poses a growing public health concern due to the escalating antimicrobial resistance. We aimed to assess site-specific MG infection and its correlates and macrolide and fluoroquinolones mutations among men who have sex with men (MSM) in Shenzhen, China. Methods: Samples were obtained from different anatomic sites of MSM based on their sexual behavior. MG infection was detected using nested polymerase chain reaction (nested PCR). Identifying macrolide and fluoroquinolone resistance involved targeting the V region of the 23S rRNA, topoisomerase IV and DNA gyrase genes. Logistic regression was used to evaluate correlates of MG infection. Results: We collected 124 pharynx swabs, 132 urethral swabs, and 89 rectal swabs from 162 MSM participants based on their sexual behavior. MG was detected in 13.0% (21/162) of MSM. The prevalence of MG in the pharynx, urethra, and rectum was 9.7% (12/124), 6.1% (8/132), and 7.9% (7/89), respectively. Among the 21 MG-positive participants, 4.8% (1/21) were infected at all three sites, and 19.0% (4/21) were infected at two sites. Of the 27 MG-positive specimens, 22.2% (2/9) exhibited mutations at position A2071G, with A2071T being the predominant mutation in the 23S rRNA gene, accounting for 77.8% (7/9) of cases. Mutations in the parC and gyrA genes were detected in 33.3% (1/3) and 33.3% (2/6) of specimens, respectively. Conclusion: We observed a high prevalence of MG infections at different anatomic sites am...