Diagnostic value and fracture healing-preventing effect of upregulated microRNA-4534 in patients with osteoporotic fractures

作者：Xufeng Chen, Chao Tang, Di-Xin Cai, Qing Yin, Qian Xie, Ping Xu, Lina Huang · 发表于：Journal of the Formosan Medical Association · 年份：2025 · DOI：10.1016/j.jfma.2025.01.003 · 被引用次数：7 · 研究领域：MicroRNA in disease regulation、Bone health and osteoporosis research、Bone Metabolism and Diseases

BACKGROUND: Osteoporosis fracture is a common and most serious complication of osteoporosis. HYPOTHESIS: This study sought to assess the level, the diagnostic potential, and the effect of circulating miR-4534 in osteoporotic fractures. METHODS: GSE74209 and GSE93883 were analyzed using GEO2R online tool for differentially expressed microRNAs in osteoporotic fractures. Postmenopausal women were recruited and serum samples were determined by RT-qPCR for level of miR-4534. ROC curves were plotted to evaluate the diagnostic value of miR-4534 in osteoporotic fractures. The effects of miR-4534 on hFOB 1.19 osteogenic marker levels, proliferation, and migration were measured by RT-qPCR, CCK-8 assay and Transwell assay, respectively. The target gene for miR-4534 was predicted and rescue experiments were conducted. RESULTS: miR-4534 was identified as an upregulated miR in osteoporotic fracture. Up-regulated miR-4534 had the potential to distinguish osteoporotic fracture from health, and from common osteoporosis. The up-regulated miR-4534 in hFOB 1.19 cocultured with human umbilical vein endothelial cells could inhibit cell osteogenic marker levels, proliferation and migration. CFTR was a target gene of miR-4534 and rescued the suppression of miR-4534 on hFOB 1.19 activity. CONCLUSION: MiR-4534 is a new potential diagnostic biomarker for osteoporotic fractures. MiR-4534 can regulate osteoblast differentiation, proliferation, and migration by targeting CFTR.