Real-time monitoring of ONOO⁻ in cerebral ischemia-reperfusion injury mouse models using a hydrazine-based NIR fluorescent probe

作者：Jinting Shang, Yan Yang, Yaojian Sun, Wanxia Gao, Kang Ma, Wang Chen, Xin Yu, Liping Li, Jiang Zheng, Na Zhao, Xiji Shu, Yibin Zhang · 发表于：Redox Biology · 年份：2025 · DOI：10.1016/j.redox.2025.103494 · 被引用次数：25 · 研究领域：Molecular Sensors and Ion Detection、Analytical Chemistry and Sensors、Nitric Oxide and Endothelin Effects

Accurate and selective techniques for visualizing endogenous peroxynitrite (ONOO − ) in cerebral ischemia-reperfusion injury (CIRI) models are essential for understanding its complex pathological processes. Here, we introduced a longwave fluorescent probe TJO for detecting ONOO − rapidly and sensitively, with a low detection limit of 91 nM. Furthermore, TJO exhibits excellent fluorescence imaging capabilities, enabling detailed visualization of ONOO⁻ in CIRI mice model. This highlights its potential for real-time monitoring of ONOO⁻-related pathological conditions. • TJO can selectively and quantitatively detect ONOO − in the near-infrared region. • The reaction mechanism is elucidated through theoretical calculations and LC-MS analysis. • TJO visualized ONOO − in living CIRI model mice, highlighting its potential for real-time tracking of related ONOO − pathology.