Soil phoD-harboring bacteria mediate the responses of phosphorus availability to N addition and mowing among soil aggregates

作者：Haiying Cui, Shanling Wang, Thum Guan Wei, Xuechen Yang, Xiuping Li, Mingcai Fan, Xiaochong Zhang, Wenzheng Song, Jianying Ma, Wei Sun · 发表于：Geoderma · 年份：2025 · DOI：10.1016/j.geoderma.2025.117170 · 被引用次数：14 · 研究领域：Soil Carbon and Nitrogen Dynamics、Plant nutrient uptake and metabolism、Legume Nitrogen Fixing Symbiosis

The conceptual framework of the responses and main drivers of available P to N addition and mowing in soil aggregates. Orange, dark green, and black dashed arrows indicate significantly negative, positive, and insignificant relationships. TOC, soil total organic C; Avai N, Available N; ALP, alkaline phosphatase; phoD -richness, phoD -harboring bacteria richness. Soil aggregates have three sizes: large macroaggregates (LMA, >2 mm), small macroaggregates (SMA, 0.25–2 mm) and microaggregates (MA, <0.25 mm). • N addition decreased soil available P regardless of mowing in macroaggregates. • High-level N addition increased available P in microaggregates under unmown conditions. • The diversity and gene abundance of phoD -harboring bacteria were positively related to available P in macroaggregates. • The alkaline phosphatase activity was the main driver of available P in microaggregates. Phosphorus (P), like nitrogen (N), is a major limiting nutrient for ecosystem structures and functions. Soils in grasslands commonly have limited P availability for organisms, especially under global change (i.e., N deposition) and land-use intensification (i.e., mowing or hay harvest). Soil phoD -harboring bacteria regulate P cycling and maintain P supply in soils. However, it remains unclear how P availability responds to N addition and mowing. The potential microbial mechanisms also require clarification among soil aggregates. We conducted a seven-year field experiment to investigate how N additi...