HMGB1 lactylation drives neutrophil extracellular trap formation in lactate-induced acute kidney injury

作者：Li Zhu, Qiang Zheng, Xiaodong Liu, Hao Ding, Mengqing Ma, Jiaxin Bao, Yawen Cai, Changchun Cao · 发表于：Frontiers in Immunology · 年份：2025 · DOI：10.3389/fimmu.2024.1475543 · 被引用次数：36 · 研究领域：Acute Kidney Injury Research、Neutrophil, Myeloperoxidase and Oxidative Mechanisms、Advanced Glycation End Products research

Rationale: Acute kidney injury (AKI) is a clinical syndrome associated with a multitude of conditions. Although renal replacement therapy (RRT) remains the cornerstone of treatment for advanced AKI, its implementation can potentially pose risks and may not be readily accessible across all healthcare settings and regions. Elevated lactate levels are implicated in sepsis-induced AKI; however, it remains unclear whether increased lactate directly induces AKI or elucidates the underlying mechanisms. Methods: ) administration of lactate (30 mg/kg) and low-dose LPS (2 mg/kg) for 24 h. Proteomic analysis was conducted to identify lactylated proteins in kidney tissues. Techniques such as, immunoprecipitation, western blotting and immunofluorescence were used to evaluate the levels of HMGB1 lactylation, neutrophil extracellular traps (NETs)and to assess related molecular signaling pathways. Main results: Our findings indicate that lactate serves as an independent predictor of AKI in patients with acute decompensated heart failure (ADHF). We observed that co-administration of lactate with low-dose lipopolysaccharide (LPS) resulted in lactate overproduction, which subsequently elevated serum levels of creatinine (Cre) and blood urea nitrogen (BUN). Furthermore, the combined application of lactate and low-dose LPS was shown to provoke HMGB1 lactylation within renal tissues. Notably, pretreatment with HMGB1 small interfering RNA (siRNA) effectively diminished lactate-mediated HMGB1 lactyl...