Multi-omic analysis on the molecular mechanisms of rapid growth in ‘Deqin’ alfalfa after space mutagenesis

作者：Qian Kuang, Chenggang He, Heping Huang, Hua Jiang · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-025-06060-5 · 被引用次数：10 · 研究领域：Plant Genetic and Mutation Studies、Plant Molecular Biology Research、Light effects on plants

BACKGROUND: Space-induced plant mutagenesis, driven by cosmic radiation, offers a promising approach for the selective breeding of new plant varieties. By leveraging the unique environment of outer space, we successfully induced mutagenesis in 'Deqin' alfalfa and obtained a fast-growing mutant. However, the molecular mechanisms underlying its rapid growth remain poorly unexplored. RESULTS: Comparative analyses of transcriptomics, proteomics, and hormone profiles were conducted in root, stem, and leaf tissues of both mutant and non-mutagenic materials. Targeted plant hormone showed notable increases in the levels of 3-indolebutyric, indole-3-acetic, and 3-indolepropionic acids in the mutant, with percentage increases of 33.55%, 32.49%, and 30.39%, respectively. Zeatin-riboside and dihydrozeatin riboside levels increased by 164.92% and 25.86%, while giberellin (GA) 7, GA3, and GA1 levels increased by 219.52%, 68.74%, and 40.98%. Non-mutagenic materials sprayed with exogenous 3-indolebutyric acid, zeatin-riboside, and GA7 exhibited significant growth acceleration. Transcriptomics identified 49,095 annotated genes, with 2,009, 1,889, and 1,760 upregulated and 2,082, 2,035, and 1,499 downregulated in the leaves, stems, and roots, respectively. Twenty-two genes related to plant hormone biosynthesis showed significant alterations. Screening through weighted correlation network analysis revealed ten candidate genes, four of which were associated with photosynthesis and starch and suc...