α-arbutin prevents UVA-induced skin photodamage via alleviating DNA damage and collagen degradation in NIH-3T3 cells

作者：Nan Zhao, Xin Nie, Yizhen Yan, Zhao Liu, Xueqing Chen, Peng Shu, Jiangming Zhong · 发表于：Journal of Photochemistry and Photobiology B Biology · 年份：2025 · DOI：10.1016/j.jphotobiol.2025.113100 · 被引用次数：7 · 研究领域：Skin Protection and Aging、melanin and skin pigmentation、Retinoids in leukemia and cellular processes

Ultraviolet radiation (UV) causes certain side effects to the skin, and their accumulation to a certain extent can lead to accelerated aging of the skin. Recent studies suggest that α-arbutin may be useful in various disorders such as hyperpigmentation disorders, wound healing, and antioxidant activity. However, the role of α-arbutin in skin photodamage is unclear. In this study, under UVA-induced photodamage conditions, α-arbutin treated mouse skin fibroblasts (NIH-3T3) can repair DNA damage and resist apoptosis by reducing the production of reactive oxygen species (ROS) and increasing the phosphorylation of glycogen synthase kinase 3 beta (GSK3β) to orchestra AKT/GSK3β pathway. Meanwhile, α-arbutin can also regulate collagen metabolism and facilitate the replenishment of collagen by targeting the phosphorylation of SMAD3 to mediate the TGFβ/SMAD pathway in NIH-3T3. In conclusion, we found that α-arbutin can mitigate the detrimental effects of skin photodamage induced by UVA irradiation, and provides a theoretical basis for the use of α-arbutin in the treatment of skin photodamage. Schematic representation of the mechanism of α-arbutin resistance to photodamage. • α-arbutin alleviates UVA-induced apoptosis and ROS in NIH-3 T3 cells. • α-ARBUTIN repairs UVA-Induced DNA damage by boosting GSK3β Phosphorylation. • α-arbutin reverses UVA-induced collagen loss by enhancing SMAD3 phosphorylation. • α-arbutin: a promising, safe option for UVA photodamage protection for skin fibrobl...