Preparation, structural property, and antioxidant activities of a novel pectin polysaccharide from the flowers of Hibiscus syriacus Linn.

作者：Jialong Chen, Ching‐Wei Ye, Lu Zhang, Zhiliang Xie, Jianjun Zhu, Zhi Zhang · 发表于：Frontiers in Nutrition · 年份：2025 · DOI：10.3389/fnut.2024.1524846 · 被引用次数：4 · 研究领域：Polysaccharides and Plant Cell Walls、Seaweed-derived Bioactive Compounds、Hibiscus Plant Research Studies

Introduction Oxidative stress, triggered by an imbalance between reactive oxygen species (ROS) production and cellular antioxidant defense mechanisms, is implicated in various pathological conditions. Plant-derived polysaccharides have gained significant attention as potential natural antioxidants due to their biocompatibility, biodegradability, and structural versatility. Methods This study focuses on the purification, structural characterization, and antioxidant activities of a novel pectin polysaccharide (HFPS) isolated from the flowers of Hibiscus syriacus Linn. HFPS was purified using anion-exchange chromatography. Its chemical composition, monosaccharide profile, molecular weight distribution, and structural properties were elucidated through various analytical techniques. Results HFPS exhibited a predominant acidic monosaccharide composition, with galacturonic acid as the major constituent, indicating its pectin nature. The free radical scavenging activity of HFPS against ABTS·, DPPḤ, and ·OH radicals was evaluated, demonstrating a positive correlation between its antioxidant capacity and concentration. Furthermore, HFPS effectively protected HepG2 cells against H 2 O 2 -induced oxidative stress by reducing ROS accumulation, modulating redox-related enzymes (Superoxide dismutase, Catalase, Glutathione peroxidase), and alleviating oxidative damage. Notably, HFPS upregulated the expression of antioxidant-related genes, including B-cell lymphoma-2 (Bcl-2), heme oxygenase ...