Predictive Application Value of Metagenomic Next‐Generation Sequencing in the Resistance of Carbapenem‐Resistant Enterobacteriaceae

作者：Jiacheng Tian, Chengtan Wang, Pingping Song, Zhiqing You, Xiuqin Jia, Xuan Li, Pang Feng · 发表于：Canadian Journal of Infectious Diseases and Medical Microbiology · 年份：2025 · DOI：10.1155/cjid/6619016 · 被引用次数：3 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial Identification and Susceptibility Testing、Genomics and Phylogenetic Studies

Objective: Although metagenomic next‐generation sequencing (mNGS) technology has achieved notable outcomes in pathogen detection, there remains a gap in the research regarding its application in predicting the antibiotic resistance of pathogenic bacteria. This study aims to analyze the clinical application value of mNGS in predicting the resistance of carbapenem‐resistant Enterobacteriaceae (CRE), as well as the relevant influencing factors, thereby providing valuable insights for clinical antimicrobial therapy. Methods: Nonduplicate isolates of Enterobacterales bacteria collected from Liaocheng People’s Hospital from April 2023 to June 2024 were selected, and CRE bacteria were screened. mNGS was used to detect resistance genes, and the results were compared with those of polymerase chain reaction (PCR) to evaluate the specificity and sensitivity of gene detection. Furthermore, the performance of mNGS in identifying pathogenic microorganisms and predicting antibiotic resistance was assessed by comparing the sequencing results with those of antimicrobial susceptibility testing (AST). Results: A total of 46 isolates were confirmed as CRE through traditional AST and were further identified using the Vitek MS and Vitek 2 systems. The results indicated 27 isolates of Klebsiella pneumoniae , 14 isolates of Escherichia coli , 2 isolates of Enterobacter hormaechei , 2 isolates of Enterobacter cloacae , and 1 isolate of Citrobacter freundii . These isolates were subjected to both mNGS...