Highly‐Efficient Differentiation of Reactive Lymphocytes in Peripheral Blood Using Multi‐Object Detection Network With Large Kernels

作者：Zihan Liu, Haoran Peng, Zhaoyi Ye, Chao Lian, Hui Shen, Hengyang Xiang, Bei Xiong, Liye Mei · 发表于：Microscopy Research and Technique · 年份：2025 · DOI：10.1002/jemt.24775 · 被引用次数：7 · 研究领域：Digital Imaging for Blood Diseases、Immunotherapy and Immune Responses、COVID-19 diagnosis using AI

Reactive lymphocytes are an important type of leukocytes, which are morphologically transformed from lymphocytes. The increase in these cells is usually a sign of certain virus infections, so their detection plays an important role in the fight against diseases. Manual detection of reactive lymphocytes is undoubtedly time-consuming and labor-intensive, requiring a high level of professional knowledge. Therefore, it is highly necessary to conduct research into computer-assisted diagnosis. With the development of deep learning technology in the field of computer vision, more and more models are being applied in the field of medical imaging. We aim to propose an advanced multi-object detection network and apply it to practical medical scenarios of reactive lymphocyte detection and other leukocyte detection. First, we introduce a space-to-depth convolution (SPD-Conv), which enhances the model's ability to detect dense small objects. Next, we design a dynamic large kernel attention (DLKA) mechanism, enabling the model to better model the context of various cells in clinical scenarios. Lastly, we introduce a brand-new feature fusion network, the asymptotic feature pyramid network (AFPN), which strengthens the model's ability to fuse multi-scale features. Our model ultimately achieves mAP50 of 0.918 for reactive lymphocyte detection and 0.907 for all leukocytes, while also demonstrating good interpretability. In addition, we propose a new peripheral blood cell dataset, providing dat...