Regional antimicrobial resistance gene flow among the One Health sectors in China

作者：Yuqing Feng, Xin Lu, Jiayong Zhao, Hongmin Li, Jialiang Xu, Zhenpeng Li, Mengyu Wang, Yao Peng, Tian Tian, Gailing Yuan, Yuan Zhang, Yuan Zhang, Jiaqi Liu, Meihong Zhang, A La Teng Zhu La, Yujiao Mu, Yujiao Mu, Wanshen Guo, Yongning Wu, Yuyu Zhang, Yuyu Zhang, Dexiang Wang, Yongfei Hu, Biao Kan · 发表于：Microbiome · 年份：2025 · DOI：10.1186/s40168-024-01983-x · 被引用次数：36 · 研究领域：Pharmaceutical and Antibiotic Environmental Impacts、Antibiotic Resistance in Bacteria、Antibiotic Use and Resistance

BACKGROUND: Antimicrobial resistance poses a significant threat to global health, with its spread intricately linked across human, animal, and environmental sectors. Revealing the antimicrobial resistance gene (ARG) flow among the One Health sectors is essential for better control of antimicrobial resistance. RESULTS: In this study, we investigated regional ARG transmission among humans, food, and the environment in Dengfeng, Henan Province, China by combining large-scale metagenomic sequencing with culturing of resistant bacterial isolates in 592 samples. A total of 40 ARG types and 743 ARG subtypes were identified, with a predominance of multidrug resistance genes. Compared with microbes from human fecal samples, those from food and environmental samples showed a significantly higher load of ARGs. We revealed that dietary habits and occupational exposure significantly affect ARG abundance. Pseudomonadota, particularly Enterobacteriaceae, were identified as the main ARG carriers shaping the resistome. The resistome in food samples was found more affected by mobile genetic elements (MGEs), whereas in environmental samples, it was more associated with the microbial composition. We evidenced that horizontal gene transfer (HGT) mediated by plasmids and phages, together with strain transmission, particularly those associated with the Enterobacteriaceae members, drive regional ARG flow. Lifestyle, dietary habits, and occupational exposure are all correlated with ARG dissemination ...