The StbHLH47 transcription factor negatively regulates drought tolerance in potato (Solanum tuberosum L.)

作者：Peijie Wang, Xiaojuan Wu, Nan Li, Hushuai Nie, Yu Ma, Yu Ma, Juan Wu, Zhicheng Zhang, Yanhong Ma, Yanhong Ma · 发表于：BMC Plant Biology · 年份：2025 · DOI：10.1186/s12870-024-06010-7 · 被引用次数：12 · 研究领域：Plant Gene Expression Analysis、Plant Stress Responses and Tolerance、Plant Molecular Biology Research

BACKGROUND: Drought stress is a major environmental constraint affecting crop yields. Plants in agricultural and natural environments have developed various mechanisms to cope with drought stress. Identifying genes associated with drought stress tolerance in potato and elucidating their regulatory mechanisms is crucial for the breeding of new potato germplasms. The bHLH transcription factors involved play crucial roles not only in plant development and growth but also in responsesresponse to abiotic stress. RESULTS: In this study, the StbHLH47 gene, which is highly expressed in potato leaves, was cloned and isolated. Subcellular localization assays revealed that the gene StbHLH47 performs transcriptional functions in the nucleus, as evidenced by increased malondialdehyde (MDA) content and relative conductivity under drought stress. These findings indicate that overexpressing plants are more sensitive to drought stress. Differential gene expression analysis of wild-type plants (WT) and plants overexpressing StbHLH47 (OE-StbHLH47) under drought stress revealed that the significantly differentially expressed genes were enriched in metabolic pathways, biosynthesis of various plant secondary metabolites, biosynthesis of metabolites, plant hormone signal transduction, mitogen-activated protein kinase (MAPK) signalling pathway-plant, phenylpropanoid biosynthesis, and plant‒pathogen interactions. Among these pathways, the phenylalanine and abscisic acid (ABA) signal transduction path...