Transcriptomic Profiles in Nasal Epithelium and Asthma Endotypes in Youth

作者：Molin Yue, K.M. Gaietto, Yueh Ying Han, F.J. Rosser, Zhongli Xu, C. Qoyawayma, Edna Acosta‐Pérez, Glorisa Canino, Erick Forno, Wei Chen, Juan C. Celedón · 发表于：JAMA · 年份：2025 · DOI：10.1001/jama.2024.22684 · 被引用次数：45 · 研究领域：Asthma and respiratory diseases、IL-33, ST2, and ILC Pathways、Delphi Technique in Research

Importance: T helper 2 (T2) cells and T helper 17 (T17) cells are CD4+ T cell subtypes involved in asthma. Characterizing asthma endotypes based on these cell types in diverse groups is important for developing effective therapies for youths with asthma. Objective: To identify asthma endotypes in school-aged youths aged 6 to 20 years by examining the distribution and characteristics of transcriptomic profiles in nasal epithelium. Design, Setting, and Participants: Cross-sectional analysis of nasal epithelial samples from 3 studies of youths with asthma aged 6 to 20 years: Stress and Treatment Response in Puerto Rican and African American Children with Asthma (STAR; N = 156), Epigenetic Variation and Childhood Asthma in Puerto Ricans (EVA-PR; N = 237), and Vitamin D Kids Asthma (VDKA; N = 66). Main Outcomes and Measures: The primary outcome was nasal epithelial transcription profiles of 3 T2 and 5 T17 pathway genes. Clinical characteristics, total and allergen-specific immunoglobulin E (IgE), blood eosinophils, and lung function were compared across profiles in all studies. Results: Mean ages for STAR, EVA-PR, and VDKA participants were 14.2, 15.4, and 10.3 years, respectively. The percentage of female participants ranged from 41% to 53.2% across studies. The predominant race or ethnicity was Puerto Rican in EVA-PR (100%) and Black or African American in STAR (71.8%) and VDKA (57.6%). Three transcriptomic profiles were identified: high T2 expression (T2HIGH), high T17 expressi...