ITGB4/CD104 mediates zika virus attachment and infection

作者：Haolong Cong, Jiuqiang Wang, Ning Du, Lei Song, Ruigang Wang, Yang Yang, Rong Lei, Tie-Shan Tang, Chang‐Mei Liu, Shuifang Zhu, Xiaodong Han · 发表于：Nature Communications · 年份：2024 · DOI：10.1038/s41467-024-54479-5 · 被引用次数：7 · 研究领域：Mosquito-borne diseases and control、Viral Infections and Vectors、Virology and Viral Diseases

Zika virus (ZIKV) infection can result in a birth defect of the brain called microcephaly and other severe fetal brain defects. ZIKV enters the susceptible host cells by endocytosis, which is mediated by the interaction of the envelope (E) glycoprotein with cellular surface receptor molecules. However, the cellular factors that used by the ZIKV to gain access to host cells remains elusive. Here, we report that the extracellular domain of integrin beta 4 (ITGB4) is an entry factor of ZIKV. ITGB4 mediates ZIKV infection by directly interacting with the E glycoprotein of ZIKV, and ITGB4 knockout hampers the binding and replication of ZIKV to host cells. A functional monoclonal antibody against ITGB4 or the soluble forms of ITGB4 could decrease the binding and infection of ZIKV to permissive cell lines. Importantly, the ITGB4 antibody blocks the infection of ZIKV to mouse placenta, thus protecting the fetuses from ZIKV infection. Together, our study has demonstrated that ZIKV infection involves ITGB4 dependent binding. Here, the authors identify integrin beta 4 (ITGB4) as a key entry factor for Zika virus infection. An antibody targeting ITGB4 protects against viral infection and prevents fetal damage in mice, offering a potential therapeutic strategy.