Fullerenol nanoparticles and AMF application for optimization of Brassica napus L. resilience to lead toxicity through physio-biochemical and antioxidative modulations

作者：Anis Ali Shah, Sheeraz Usman, Zahra Noreen, Muhammad Kaleem, Vaseem Raja, Mohamed A. El‐Sheikh, Zakir Ibrahim, Shafaque Sehar · 发表于：Scientific Reports · 年份：2024 · DOI：10.1038/s41598-024-82086-3 · 被引用次数：19 · 研究领域：Nanoparticles: synthesis and applications、Aluminum toxicity and tolerance in plants and animals、Plant Stress Responses and Tolerance

Crop plants are severely affected by heavy metals (HMs), leading to food scarcity and economical loss. Lead (Pb) is outsourced by use of lead-based fertilizers, batteries, mining, smelting and metal processing. It significantly reduces growth, development and yield of crops cultivated on contaminated sites. In this study, the ameliorative role of carbon based fullerenol nanoparticles (FNPs) in combination with Arbuscular Mycorrhizal Fungi (AMF) inoculation was examined in Brassica napus L. grown in Pb contaminated soil. A pot experiment in three-ways completely randomize fashion with three replicates was conducted. For Pb stress, a 200 µM PbCl 2 solution was applied at a rate of 1 L per pot. FNPs were applied via foliar spray at a concentration of 3 mM. For AMF inoculation rhizospheric soil was collected from Sorghum bicolor fields and used in this experiment. Results of the study showed that Pb toxicity greatly reduced growth (shoot length; 15%, root length; 25%) of B. napus plants. It lowered photosynthesis (38%) and gas exchange related attributes. Pb contamination caused oxidative stress, evident from elevated level of malondialdehyde (62%), and reactive oxygen species (H 2 O 2 ; 60%, OH − ; 103% and O 2 •− ; 23%). It also triggered the antioxidant defense system of B. napus . These plants also had high Pb metal ions in their root and shoot compared with control. Foliar application of FNPs along with AMF inoculation effectively mitigated oxidative stress caused by Pb via ...