Electrochemical Glucose Sensor Based on Dual Redox Mediators

作者：Changyun Quan, Yue Zhang, Yuanyuan Liu, Liping Wen, Haixia Yang, Xueqin Huang, Minghui Yang, Binjie Xu · 发表于：Biosensors · 年份：2024 · DOI：10.3390/bios15010009 · 被引用次数：14 · 研究领域：Electrochemical sensors and biosensors、Analytical Chemistry and Sensors、Advanced Chemical Sensor Technologies

Electrochemical glucose sensor holds significant promise for the monitoring of blood glucose levels in diabetic patients. In this study, we proposed a novel electrochemical glucose sensor based on 1,10-Phenanthroline-5,6-dione (PD)/Ru(III) as a dual redox mediator. The synergistic effect of PD and Ru(III) was utilized to efficiently facilitate the electron transfer between the enzyme-active center and the electrode. Then, a commercial disposable electrochemical glucose sensor was constructed based on screen-printing electrodes. Experimental results indicated the synergy between PD and Ru(III) provided a promising electron transfer environment for a glucose dehydrogenase (GDH)-catalyzed glucose reaction. The sensor exhibits a linear glucose response range from 0.01 to 38.6 mmol/L, with a limit of detection (LOD) as low as 7.0 µmol/L and a sensitivity of 38 µA·L/(mmol·cm2). The accuracy of the sensor was further validated in spiked recovery tests of human venous blood samples. The glucose recovery rate was between 99.5% and 107%, with a relative standard deviation (RSD) of less than 3.2%. These results demonstrate that our sensor has high potential for commercialization and practical application in glucose monitoring.