Lonicera caerulea genome reveals molecular mechanisms of freezing tolerance and anthocyanin biosynthesis

作者：Jing Wang, Xiaotong Wang, Yuwei Ma, Ranran Gao, Yongmiao Wang, Zhoujie An, Ya Tian, Huihua Wan, Dianwen Wei, Feng Wang, Baojiang Zheng, Baozhong Duan, Xiang Li, Gangqiang Dong, Wei Sun, Zhichao Xu · 发表于：Journal of Advanced Research · 年份：2024 · DOI：10.1016/j.jare.2024.12.038 · 被引用次数：10 · 研究领域：Plant Gene Expression Analysis、Phytochemicals and Antioxidant Activities、Plant Molecular Biology Research

INTRODUCTION: Lonicera caerulea L. (blue honeysuckle) is a noteworthy fleshy-fruited tree and a prominent medicinal plant, which possesses notable characteristics such as exceptional resilience to winter conditions and early maturation, and the richest source of functional anthocyanins, particularly cyanidin-3-glucoside. The molecular mechanisms responsible for its freezing tolerance and anthocyanin biosynthesis remain largely unknown. OBJECTIVES: Here, a chromosome-scale genome of L. caerulea was presented, aiming to examine the genetic foundations that underlie these characteristics of blue honeysuckle. METHODS: The PacBio HiFi reads and Hi-C data were used to construct high-quality genome of blue honeysuckle. Comparative genomic and transcriptomic analyses were conducted to elucidate the molecular mechanisms of freezing tolerance and anthocyanin biosynthesis. RESULTS: Comparative genomics analysis between L. caerulea and L. japonica revealed that the dynamic changes of duplicated genes contributed to their phytochemical reconstruction and environmental adaptation. Moreover, the ABA and ICE-CBF-COR signaling pathways were closely correlated to the freezing tolerance of L. caerulea. Genome-wide identification and biochemical function indicated that three anthocyanin 3',5'-O-methyltransferases (LcOMT2, LcOMT14, and LcOMT20) and two 3'-O-glycosyltransferases (LcUGT78X1 and LcUGT95P1) were responsible for anthocyanin biosynthesis. In addition, LcUGT78X1 was regarded as the pote...