Dual-Amplification Single-Particle ICP-MS Strategy Based on Strand Displacement Amplification–CRISPR/Cas12a Amplification for Homogeneous Detection of miRNA

作者：Yan Xu, Beibei Chen, Man He, Guolin Yuan, Bin Hu · 发表于：Analytical Chemistry · 年份：2024 · DOI：10.1021/acs.analchem.4c05385 · 被引用次数：31 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA Interference and Gene Delivery、MicroRNA in disease regulation

MicroRNAs (miRNAs) regulate a myriad of biological processes and thus have been regarded as useful biomarkers in biomedical research and clinical diagnosis. The specific and highly sensitive detection of miRNAs is of significant importance. Herein, a sensitive and rapid dual-amplification elemental labeling single-particle inductively coupled plasma-mass spectrometry (spICP-MS) analytical method based on strand displacement amplification (SDA) and CRISPR/Cas12a was developed for miRNA-21 detection. Taking gold nanoparticles (AuNPs) as the elemental labels, the Au NP probe initially hybridized with linker DNA, forming large aggregates. In the absence of target miRNA-21, large aggregates of AuNPs will produce high pulse signals in spICP-MS detection. In the presence of the target miRNA-21, it triggered the SDA reaction, and the SDA products activated CRISPR/Cas12a’s trans-cleavage activity to cleave the linker DNA, resulting in disassembly of the AuNP aggregates. The AuNP aggregates with smaller size displayed lower pulse signals in spICP-MS detection. Under the optimal conditions, a good relationship between the average pulse signal intensity of AuNP aggregates and the concentration of miRNA-21 was obtained in the range of 0.5 fmol L –1 –100 pmol L –1 with a quantification limit as low as 0.5 fmol L –1 . The developed method was successfully used for determination of miRNA-21 in human breast cancer cell lines (SK-BR-3 and MCF-7) and real blood samples from breast cancer patien...