Urbanization enhances consumer protist-driven ARGs dissemination in riverine ecosystems

作者：Bob Adyari, Lanping Zhang, Ana Maravić, Jiaxin Chen, Laiyi Li, Mahmoud Gad, Chang‐Ping Yu, Anyi Hu · 发表于：Environment International · 年份：2024 · DOI：10.1016/j.envint.2024.109238 · 被引用次数：7 · 研究领域：CRISPR and Genetic Engineering、Prion Diseases and Protein Misfolding、Insect symbiosis and bacterial influences

• Watershed urbanization increased the abundance and richness of riverine ARGs. • Mobile ARGs reflected the urbanization better than non-mobile ARGs. • Urbanization boosted shared ARGs between pathogen and non-pathogen contigs. • Consumer protists drove ARG dissemination in highly urbanized tributary. • Micro-eukaryotic algae drove ARG dissemination in less urbanized tributary. Despite the emergence of antibiotic resistance genes (ARGs) and antibiotic resistant bacteria (ARBs), how biological inter-trophic interactions, modulated by watershed urbanization, shape the resistome remains unexplored. We collected water samples from the highly urbanized (western: 65 % built land, sewage-affected) and lesser-urbanized (northern: 25 % built land, drinking water source) downstream tributaries of the Jiulong River in southeast China over dry and wet seasons. We utilized metagenomic and amplicon (16S and 18S rDNA) sequencing to investigate the relationships among microeukaryotic algae, consumer protists, bacterial communities, and the resistome. Metagenomic results showed that ARG-MGE-carrying contigs (mobile ARGs), rather than ARG-carrying contigs (non-mobile ARGs), exhibited more pronounced discrepancies between tributaries. A higher total abundance of ARGs and a greater number of co-shared ARGs between pathogen and non-pathogen bacteria were observed in the more urbanized western tributary. Structural equation modeling revealed that consumer protist-bacteria and algae-bacteria cohesi...