A guide to selecting high-performing antibodies for STXBP1 (UniProt ID: P61764) for use in western blot, immunoprecipitation, and immunofluorescence

作者：Riham Ayoubi, Charles Alende, Maryam Fotouhi, Sara González Bolívar, Carl Laflamme, NeuroSGC/YCharOS/EDDU collaborative group · 发表于：F1000Research · 年份：2024 · DOI：10.12688/f1000research.160174.1 · 被引用次数：1 · 研究领域：Cellular transport and secretion、Lipid Membrane Structure and Behavior、Neuroscience and Neuropharmacology Research

The Syntaxin-binding protein 1, STXBP1, is a protein involved in docking and fusion of synaptic vesicles, a crucial event for neurotransmitters release into the synapse. Here we have characterized twelve STXBP1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. These studies are part of a larger, collaborative initiative seeking to address antibody reproducibility issues by characterizing commercially available antibodies for human proteins and publishing the results openly as a resource for the scientific community. While use of antibodies and protocols vary between laboratories, we encourage readers to use this report as a guide to select the most appropriate antibodies for their specific needs.