Analysis of the molecular mechanism endogenous hormone regulating axillary bud development in Pinus yunnanensis

作者：Haihao He, Junfei Xu, Nianhui Cai, Yulan Xu · 发表于：BMC Plant Biology · 年份：2024 · DOI：10.1186/s12870-024-05819-6 · 被引用次数：10 · 研究领域：Plant Molecular Biology Research、Plant Gene Expression Analysis、Plant Parasitism and Resistance

BACKGROUND: P. yunnanensis, a distinctive economic tree species native to Yunnan Province in China, possesses axillary buds that serve as superior material for asexual propagation. However, under natural growth conditions, the differentiation of these axillary buds is notably scarce. In this study, we employed decapitation to stimulate the development of axillary buds in P. yunnanensis. Subsequently, we assessed the phytohormone levels in both axillary and apical buds, and conducted a comprehensive transcriptomic analysis complemented by RT-qPCR validation. RESULTS: We found that decapitation could effectively promote the releases of the axillary buds in P. yunnanensis. The levels of cytokinin, auxin, gibberellin and abscisic acid in axillary buds were higher than those in apical buds, and the difference in gibberellin levels was the greatest. The transcriptome sequencing results were highly reproducible, and the relative expression levels of the 13 genes screened were highly consistent with the FPKM value trend of transcriptome sequencing. There were 2877 differentially expressed genes (DEGs) between axillary buds and terminal buds, and 18 candidate genes (CGs) involved in axillary bud release were screened out. A total of 1171 DEGs were identified during the analysis of axillary bud growth, and 14 CGs involved in axillary bud growth and development were screened out. GO and KEGG enrichment analysis were performed on the DEGs. Furthermore, combined with the results and discu...