High prevalence and plasmidome diversity of optrA-positive enterococci in a Shenzhen community, China

作者：Yulin Fu, Zhaoju Deng, Yingbo Shen, Weizhou Wei, Qiumei Xiang, Zhiyang Liu, Kunning Hanf, Suli Huang, Zhongcheng Lv, Zhongcheng Lv, Tingting Cao, Changfeng Peng, Rong Zhang, Xuan Zou, Jianzhong Shen, Štefan Schwarz, Yang Wang, Dejun Liu, Ziquan Lv, Ziquan Lv, Yuebin Ke · 发表于：Frontiers in Microbiology · 年份：2024 · DOI：10.3389/fmicb.2024.1505107 · 被引用次数：7 · 研究领域：Bacteriophages and microbial interactions、Antibiotic Resistance in Bacteria、Microbial infections and disease research

Background The emergence of optrA , which can confer resistance to phenicols and oxazolidinones in Enterococcus spp., poses a growing public health threat. Methods 102 optrA -positive enterococci (OPEs) including various species were isolated from feces of 719 healthy volunteers in a Shenzhen community, China. Antimicrobial susceptibility of these isolates was tested. Whole-genome sequencing and bioinformatics analysis were performed to characterize molecular epidemiology of OPEs. Results Compared to optrA -negative enterococci (ONEs), antimicrobial resistance (linezolid, florfenicol, doxycycline, erythromycin and ciprofloxacin) and presence of antimicrobial resistance genes (ARGs) ( fexA , cat , tet (M), erm (A), erm (B) and etc) were higher in OPEs. Phylogenetic analysis revealed that high similarly (19–338 SNPs) was observed between the optrA -positive E. faecalis from community and the strains from patients, animals, and environment. In 102 OPEs, the optrA gene was detected on the chromosome ( n = 36), on plasmids ( n = 62), or both ( n = 4). A diverse range of optrA -carrying plasmid types was identified. The rep 9-plasmid replicons were widely detected in E. faecalis (44/66), whereas rep US1-plasmid replicons were widely identified in other enterococcal species (7/66). Most of all ARGs harbored by isolates were co-existed on optrA -carrying plasmids, suggesting that the acquisition of optrA -carrying plasmids will pose a greater threat to public health. Notably, the pAD...