Inoculation with Micromonospora sp. enhances carbohydrate and amino acid production, strengthening antioxidant metabolism to mitigate heat stress in wheat cultivars

作者：Abdelrahim H. A. Hassan, Enas Shaban Ahmed, Mohamed S. Sheteiwy, Yousef Alhaj Hamoud, Mohammad K. Okla, Amal Mohamed AlGarawi, María Gabriela Maridueña-Zavala, Ibrahim A. Alaraidh, Ahmed Mohamed Reyad, Hamada AbdElgawad · 发表于：Frontiers in Plant Science · 年份：2024 · DOI：10.3389/fpls.2024.1500894 · 被引用次数：8 · 研究领域：Plant Stress Responses and Tolerance、Plant responses to elevated CO2、Seed Germination and Physiology

Introduction Heat stress caused by global warming adversely affects wheat yield through declining most nutritional quality attributes in grains, excluding grain protein content. Methods This research investigated the biochemical, physiological, and antioxidant responses of wheat plants under heat stress, focusing on the role of plant growth-promoting bacteria ( Micromonospora sp.). Two wheat genotypes were studied: one heat-sensitive and one heat-tolerant, examining their responses to heat stress with and without bacterial inoculation. Results Under heat stress, the sensitive cultivar experienced significant reductions in photosynthesis rate, chlorophyll content, and RuBisCO activity (57-61%), while the tolerant cultivar had milder reductions (24-28%). Micromonospora sp. treatment notably improved these parameters in the sensitive cultivar (+48-78%), resulting in a substantial increase in biomass production (+43-53%), which was not seen in the tolerant cultivar. Additionally, oxidative stress markers (H 2 O 2 and MDA) were elevated more in the sensitive cultivar (82% and 90% higher) compared to the tolerant one. Micromonospora sp. treatment effectively reduced these markers in the sensitive cultivar (-28% and -27%). Enhanced activity of antioxidant enzymes and ASC-GSH pathway enzymes was particularly evident in Micromonospora sp.-treated sensitive plants. Carbohydrate metabolism shifted, with increased soluble sugars and significant rises in sucrose content in Micromonospora ...