Cannabidiol alleviates LPS ‐inhibited odonto/osteogenic differentiation in human dental pulp stem cells in vitro

作者：Chatvadee Kornsuthisopon, Ajjima Chansaenroj, Ravipha Suwittayarak, Suphalak Phothichailert, Khunakon Usarprom, Apicha Srikacha, Sornkanok Vimolmangkang, Chaloemrit Phrueksotsai, Lakshman Perera Samaranayake, Thanaphum Noom Osathanon · 发表于：International Endodontic Journal · 年份：2024 · DOI：10.1111/iej.14183 · 被引用次数：12 · 研究领域：Cannabis and Cannabinoid Research、Mesenchymal stem cell research、Autophagy in Disease and Therapy

AIM: Cannabidiol (CBD), derived from the Cannabis sativa plant, exhibits benefits in potentially alleviating a number of oral and dental pathoses, including pulpitis and periodontal diseases. This study aimed to explore the impact of CBD on several traits of human dental pulp stem cells (hDPSC), such as their proliferation, apoptosis, migration and odonto/osteogenic differentiation. METHODOLOGY: hDPSCs were harvested from human dental pulp tissues. The cells were treated with CBD at concentrations of 1.25, 2.5, 5, 10, 25 and 50 μg/mL. Cell responses in terms of cell proliferation, colony-forming unit, cell cycle progression, cell migration, apoptosis and odonto/osteogenic differentiation of hDPSCs were assessed in the normal culture condition and P. gingivalis lipopolysaccharide (LPS)-induced 'inflammatory' milieus. RNA sequencing and proteomic analysis were performed to predict target pathways impacted by CBD. RESULTS: CBD minimally affects hDPSCs' behaviour under normal culture growth milieu in normal conditions. However, an optimal concentration of 1.25 μg/mL CBD significantly countered the harmful effects of LPS, indicated by the promoting cell proliferation and restoring the odonto/osteogenic differentiation potential of hDPSCs under LPS-treated conditions. The proteomic analysis demonstrated that several proteins involved in cell proliferation and differentiation were upregulated following CBD exposure, including CCL8, CDC42 and KFL5. RNA sequencing data indicated that ...