Acinetobacter indicus Coharboring Tet(X6) and blaNDM-1 Isolated From Slaughterhouse Waste

作者：Xiaoqian Long, Feng Lin, Biao Tang, Fengcheng Miao, Zhiyu Li, Yao Shen, Hua Yang, Jian‐Gang Ma · 发表于：Journal of Global Antimicrobial Resistance · 年份：2024 · DOI：10.1016/j.jgar.2024.12.004 · 被引用次数：6 · 研究领域：Antibiotic Resistance in Bacteria、Bacterial biofilms and quorum sensing、Bacillus and Francisella bacterial research

• Acinetobacter indicus isolated from water carries bla NDM-1 and tet (X6) genes. • bla NDM-1 located in the complex transposon Tn 125. • tet (X6) on the chromosome transferred by IS CR2. Acinetobacter indicus is an important pathogen of nosocomial infection. The purpose of this study was to analyze the resistance and transmission of A. indicus strain AIBD14 isolated from slaughterhouse environment. A total of 96 environmental samples were collected from slaughterhouse. The antimicrobial susceptibility test was carried out by microbroth dilution method and E -test. Whole genome sequencing and bioinformatics analysis of the AIBD14 were performed, then S1-PFGE and southern blot verified the location of bla NDM-1 and tet (X6). The AIBD14 is resistant to meropenem but susceptibility to tigecycline, and coharboring bla NDM-1 and tet (X6). The bla NDM-1 is located on the pAIBD14-NDM-1 that cannot be transferred by conjugation. Specifically, bla NDM-1 is located on the transposon Tn 125 , and bla NDM-1 can be transferred to other species with the help of transposon. The genetic background of bla NDM-1 is "IS Aba125 - bla NDM-1 - ble MEL - dsbD - cutA - groES - groEL - insE -IS Aba125 ". pAIBD14-NDM-1 is classified into the GR31 plasmid based on the homology of the repB . Meanwhile, there are two XerC/D-like binding sites on the plasmid, which can mediate the transfer of resistance genes. The tet (X6) gene is located on the chromosome of AIBD14, its downstream is accompanied by the n...