Electrochemical immunoassay for gastric cancer biomarker pepsinogen I detection based on PdAgPt/MoS 2

作者：Chunsheng He, Zhisong Qiu, Feng Jin, Lifang Weng, Libin Chen, Lijuan Wang, Sicong Jiang, Jin Shi · 发表于：Biomedical Materials · 年份：2024 · DOI：10.1088/1748-605x/ad9fc7 · 被引用次数：4 · 研究领域：Advanced biosensing and bioanalysis techniques、RNA and protein synthesis mechanisms、Acoustic Wave Resonator Technologies

Abstract This study presents a novel electrochemical immunosensor for the detection of pepsinogen I, a potential biomarker for gastric cancer, based on a unique PdAgPt/MoS 2 nanocomposite. The key innovation lies in the synergistic combination of trimetallic PdAgPt nanoparticles with MoS 2 nanoflowers, which has not been previously reported for pepsinogen I detection. This hybrid material demonstrates exceptional electron transfer properties and a significantly larger electroactive surface area compared to conventional materials. The optimized immunosensor exhibits superior performance metrics: a wide linear range of 0.5–200 ng ml −1 and an unprecedented low detection limit of 0.173 ng ml −1 , surpassing existing detection methods. The sensor shows remarkable selectivity with interfering substances exhibiting relative responses below 5%, excellent reproducibility (RSD 3.8%), and outstanding stability (95.6% retention after 30 d). Analysis of spiked serum samples resulted in recoveries ranging from 96.8% to 104.5%, demonstrating the sensor’s practical applicability for early gastric cancer screening. This work represents a significant advancement in developing rapid, sensitive, and cost-effective diagnostic tools for gastric cancer surveillance.