Effects of direct moxibustion on antigen-presenting cells in gastric tissue of rat models with gastric cancer: Understanding the immunological mechanisms

作者：Tianyu Gao, Fei-cheng ZHANG, Ru-fang PAN, Shu-ya MA, Xiaoqi Zhang, Lijia Pan, Chun‐Sheng Jia · 发表于：世界针灸杂志：英文版 · 年份：2024 · DOI：10.1016/j.wjam.2024.12.004 · 被引用次数：2 · 研究领域：Acupuncture Treatment Research Studies、Cancer, Stress, Anesthesia, and Immune Response、Healthcare and Venom Research

The preventive and therapeutic effects of direct moxibustion on a gastric cancer rat model induced by the intragastric administration of N -methyl- N '-nitro- N -nitrosoguanidine (MNNG) were evaluated. Changes in the co-stimulatory molecules CD80 and CD86 on antigen-presenting cells in gastric tissues as well as related cytokines in serum were evaluated. The aim of the study was to explore the immunological mechanisms by which direct moxibustion may prevent gastric cancer lesions, thereby providing a basis for studies on the immunological mechanisms by which moxibustion prevents tumor development. Sixty healthy male Wistar rats were randomly divided into four groups: Control, control+moxibustion, model, and moxibustion groups. A gastric cancer rat model was induced by intragastric administration of 20 mg/mL MNNG, with a dose of 1 mL/100 g body weight, once daily for 16 weeks. The control+moxibustion and moxibustion groups received direct moxibustion simultaneously with modeling, continuing for 16 weeks. After the experiment, gastric tissue was collected, and morphological changes in the gastric mucosa in each group of rats were observed through H&E staining. Immunohistochemistry (IHC) and a western blotting were used to detect the expression levels of CD80 and CD86 in gastric tissues. Enzyme-linked immunosorbent assays (ELISA) were used to measure the levels of interleukin-12 (IL-12), interferon-gamma (IFN-γ), and tumor necrosis factor-beta (TNF-β) in rat serum. Upon macrosco...