Kiwifruit spatiotemporal multiomics networks uncover key tissue-specific regulatory processes throughout the life cycle

作者：Zhebin Zeng, Yawei Li, Man Zhu, Xiaoyao Wang, Wang Yan, Ang Li, Xiaoya Chen, Qianrong Han, Niels J. Nieuwenhuizen, Charles Ampomah‐Dwamena, Xiuxin Deng, Yunjiang Cheng, Qiang Xu, Xiao Cui, Fan Zhang, Ross G. Atkinson, Yunliu Zeng · 发表于：PLANT PHYSIOLOGY · 年份：2024 · DOI：10.1093/plphys/kiae567 · 被引用次数：19 · 研究领域：Plant Gene Expression Analysis、Plant biochemistry and biosynthesis、Antioxidant Activity and Oxidative Stress

Kiwifruit (Actinidia chinensis), a recently commercialized horticultural crop, is rich in various nutrient compounds. However, the regulatory networks controlling the dynamic changes in key metabolites among different tissues remain largely unknown. Here, high-resolution spatiotemporal datasets obtained by ultraperformance liquid chromatography-tandem mass spectrometry methodology and RNA-seq were employed to investigate the dynamic changes in the metabolic and transcriptional landscape of major kiwifruit tissues across different developmental stages, including from fruit skin, outer pericarp, inner pericarp, and fruit core. Kiwifruit spatiotemporal regulatory networks (KSRN) were constructed by integrating the 1,243 identified metabolites and co-expressed genes into 10 different clusters and 11 modules based on their biological functions. These networks allowed the generation of a global map for the major metabolic and transcriptional changes occurring throughout the life cycle of different kiwifruit tissues and discovery of the underlying regulatory networks. KSRN predictions confirmed previously established regulatory networks, including the spatiotemporal accumulation of anthocyanin and ascorbic acid (AsA). More importantly, the networks led to the functional characterization of three transcription factors: an A. chinensis ethylene response factor 1, which negatively controls sugar accumulation and ethylene production by perceiving the ripening signal, a basic-leucine zip...