Synchrotron-based infrared microspectroscopy unveils the biomolecular response of healthy and tumour cell lines to neon minibeam radiation therapy

作者：Roberto González-Vegas, Olivier Seksek, Annaïg Bertho, Judith Bergs, Ryoichi Hirayama, Taku Inaniwa, Naruhiro Matsufuji, Takashi Shimokawa, Yolanda Prezado, Ibraheem Yousef, Immaculada Martínez‐Rovira · 发表于：The Analyst · 年份：2024 · DOI：10.1039/d4an01038h · 被引用次数：4 · 研究领域：Spectroscopy Techniques in Biomedical and Chemical Research、Spectroscopy and Chemometric Analyses、Nuclear Physics and Applications

Radioresistant tumours remain complex to manage with current radiotherapy (RT) techniques. Heavy ion beams were proposed for their treatment given their advantageous radiobiological properties. However, previous studies with patients resulted in serious adverse effects in the surrounding healthy tissues. Heavy ion RT could therefore benefit from the tissue-sparing effects of minibeam radiation therapy (MBRT). To investigate the potential of this combination, here we assessed the biochemical response to neon MBRT (NeMBRT) through synchrotron-based Fourier transform infrared microspectroscopy (SR-FTIRM). Healthy (BJ) and tumour (B16-F10) cell lines were subjected to seamless (broad beam) neon RT (NeBB) and NeMBRT at HIMAC. SR-FTIRM measurements were conducted at the MIRAS beamline of ALBA Synchrotron. Principal component analysis (PCA) permitted to assess the biochemical effects after the irradiations and 24 hours post-irradiation for the different RT modalities and doses. For the healthy cells, NeMBRT resulted in the most dissimilar spectral signatures from non-irradiated cells early after irradiations, mainly due to protein conformational modifications. Nevertheless, most of the damage appeared to recover one day post-RT; conversely, protein- and nucleic acid-related IR bands were strongly affected by NeBB 24 hours after treatment, suggesting superior oxidative damage and nucleic acid degradation. Tumour cells appeared to be less sensitive to NeBB than to NeMBRT shortly after...