Licorice-wolfberry derived nanomaterials enhance sclerotinia stem rot resistance by activating JA-mediated immune response in rapeseed

作者：Ping Qiu, Jiahui Sun, Jiahao Liu, Zhichao Mei, Chaochun Wang, Xin Wang, Qinqin Chen, Xin He, Xinghua Xiong, Linfeng Bao, Zhongsong Liu, Honghong Wu, Yunlong Zhai, Lunwen Qian · 发表于：Industrial Crops and Products · 年份：2024 · DOI：10.1016/j.indcrop.2024.120279 · 被引用次数：4 · 研究领域：Plant pathogens and resistance mechanisms、Agronomic Practices and Intercropping Systems、Plant-Microbe Interactions and Immunity

Due to the lack of resistant germplasm, sclerotinia stem rot (SSR) caused by S. sclerotiorum ( Sclerotinia sclerotiorum ) has become the most destructive disease in rapeseed ( Brassica napus L.) production. Nanobiotechnology has recently been considered as a sustainable approach for crop protection by reducing pesticide use. In this study, a novel blue-green fluorescent nanocomplex derived from licorice and wolfberry (LW-NCs) was synthesized and characterized, exhibiting stronger antioxidant activity compared with licorice or wolfberry extracts alone. LW-NCs promoted the growth of rapeseed seedlings without affecting photosynthesis efficiency. In vitro antifungal assays revealed that LW-NCs significantly inhibited the growth of S. sclerotiorum at a concentration of 200 ppm. Transcriptomic and metabolomic analyses demonstrated that foliar application of LW-NCs increased the JA content and the activities of SOD and CAT in rapeseed, ultimately enhancing the tolerance of rapeseed to S. sclerotiorum . In addition, toxicity experiments have demonstrated that LW-NCs possess excellent biocompatibility. These results indicated that the application of LW-NCs can not only improve the disease resistance of rapeseed, but also help reduce the harm of pesticides to human health, ensuring the safety of them for agricultural applications. • A novel Licorice-Wolfberry derived nanomaterials were synthesized and characterized. • Nanomaterials promote the growth of rapeseed seedlings. • Nanomater...