Prevalence and molecular characterization of human bocavirus-1 in children and adults with influenza-like illness from Kunming, Southwest China

作者：Yanhong Sun, Lili Jiang, Yaoyao Chen, Zhao‐Sheng Liu, Meiling Zhang, Xiaonan Zhao, Xiaoyu Han, Lifen Zhang, Xiaoqing Fu, Jienan Zhou · 发表于：Microbiology Spectrum · 年份：2024 · DOI：10.1128/spectrum.01564-24 · 被引用次数：6 · 研究领域：Respiratory viral infections research、Influenza Virus Research Studies、Pneumonia and Respiratory Infections

ABSTRACT Human bocavirus-1 (HBoV-1) has been associated with respiratory infections in both children and adults, often presenting symptoms similar to those of influenza. Understanding the prevalence and molecular characteristics of HBoV-1 in individuals with influenza-like illness (ILI) is essential for enhancing the diagnosis and treatment of respiratory infections in Kunming, Southwest China. Between December 2017 and December 2023, demographic and clinical data, along with respiratory tract specimens from individuals aged 0 to 97 years with ILI, were collected at three sentinel hospitals in Kunming. Each specimen was tested for 18 respiratory viruses, and the positive rates of HBoV-1 across different age groups were analyzed. Amplification of the near-complete HBoV genome was achieved through three overlapping fragments, followed by next-generation sequencing (NGS) and phylogenetic analysis. A total of 20,181 respiratory samples were collected from patients aged 1 month to 97 years presenting with ILI symptoms between December 2017 and December 2023, with HBoV detected in 0.8% of the samples. The prevalence was 1.0% (165/16,406) in children and 0.1% (3/3,775) in adults, with a significantly higher detection rate in pediatric patients (<18 years old) compared to adults (≥18 years old) ( P < 0.001). Among the 168 HBoV-positive participants, 165 (98.2%) were children under 18 years, while 3 (1.8%) were adults. Genome-wide phylogenetic analyses indicated that HBoV-1 was ...