Downregulation of MLF1 safeguards cardiomyocytes against senescence-associated chromatin opening

作者：Jian Lv, Qin Chen, Junmei Wang, Ningning Guo, Fang Yu, Qiuxiao Guo, Jiajie Li, Xiao Ma, Hongchao Zhan, Weihao Chen, Li Wang, Qingqing Yan, Jingjing Tong, Wang Zh · 发表于：Nucleic Acids Research · 年份：2024 · DOI：10.1093/nar/gkae1176 · 被引用次数：16 · 研究领域：Telomeres, Telomerase, and Senescence、RNA Research and Splicing、RNA modifications and cancer

Aging-associated cardiac hypertrophy (AACH) increases susceptibility to heart failure in the elderly. Chromatin remodeling contributes to the gene reprogramming in AACH; however, the intrinsic regulations remain elusive. We performed a transcriptome analysis for AACH in comparison with pressure-overload-induced pathological cardiac hypertrophy in mice and identified myeloid leukemia factor 1 (MLF1) as an aging-sensitive factor whose expression was reduced during aging but could be reversed by anti-aging administrations. In human AC16 cardiomyocytes, silencing MLF1 suppressed H2O2-induced cell senescence while the phenotype was exacerbated by MLF1 overexpression. RNA-seq analysis revealed that MLF1 functioned as a transcription activator, regulating genomic-clustered genes that mainly involved in inflammation and development. ATAC-seq analysis showed a prominent reduction in chromatin accessibility at the promoter regions of senescence effectors, like IL1B and p21, after MLF1 knockdown. Despite a potential interaction of MLF1 with the histone methyltransferase PRC2, its inhibition failed to reverse the impact of MLF1 knockdown. Instead, MLF1-mediated regulation was blunted by inhibiting the acetyltransferase EP300. CUT&Tag analysis showed that MLF1 bound to target promoters and recruited EP300 to promote H3K27ac deposition. Collectively, we identify MLF1 as a pro-aging epigenetic orchestrator that recruits EP300 to facilitate opening of the condensed chromatin encompassing sen...